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3’ hydroxy group

Different classes of nucleoside/nucleotide analogs have different effects on polymerases. Remdesivir is in a class called nonobligate chain terminators, because it should, in theory, be possible to add more nucleotides to a strand of RNA after remdesivir has been added due to the presence of the hydroxyl group at carbon 3 in the sugar.

“That hydroxy group is what is required for continued synthesis of nucleic acid, whether it be RNA or DNA,” said virologist Craig Cameron, a professor at the University of North Carolina at Chapel Hill who studies the interactions between nucleoside analogs and viral polymerases.

suggests that when mixed with RNA polymerases from coronaviruses or flaviviruses in vitro, remdesivir doesn’t terminate the synthesis of a new RNA strand right away. Instead, Cameron said, “It takes a few cycles of nucleotide addition before you can see the termination effect.”

Those additional nucleotides may help shield remdesivir from coronavirus proofreading enzymes that are known to remove unnatural nucleotide analogs.

Base pairing to uracil

In adenosine in double-stranded RNA, this face of the molecule is involved in base-pairing with uracil. The two nitrogens act as proton donor and acceptor, respectively for hydrogen bonds to atoms in the uracil base.

Chemists think that remdesivir, by presenting a very similar binding face, gets incorporated into a growing RNA strand by viral polymerases.

C-nucleoside bond

The link between ribose and the base is called the glycosidic bond. Usually, it connects the 1’ carbon in the ribose ring to a nitrogen in the base. But in remdesivir (and some other nucleotide analogs) the sugar and the nucleobase are connected by a bond between two carbons.

“It definitely provides much greater stability (against) nucleases and other enzymes that can cleave the nucleobase from the sugar,” said Katherine Seley-Radtke, a medicinal chemist at the University of Maryland, Baltimore County who works on the design and synthesis of antiviral nucleoside analogues. With a C-nucleoside, “you’d have to break a carbon-carbon bond, whereas in a normal nucleoside you’re breaking a hemi-aminal bond, which is actually fairly unstable. So having that carbon-carbon bond is a great advantage.”

1’ cyano group

Ask a group of chemists what jumps out at them about remdesivir, and most will start with this dramatic feature. Substitution at this carbon is unusual, and probably only possible because of the strength of the C-nucleoside bond.

According to in the Journal of Medicinal Chemistry, the cyano group was initially added because a precursor molecule, a very effective inhibitor of viral RNA polymerases, also blocked the mitochondrial RNA polymerase in mice. To make a molecule without those toxic side effects, chemists at Gilead tried a series of substitutions at the 1’ carbon. The compound with the cyano group worked best: it still blocked the hepatitis C polymerase, but was no longer incorporated by host cell polymerases.

“You can’t predict activity. You have to make it and test it,” Seley-Radtke said. “But even small changes can have amazing consequences.”

Phosphate

“You see all that flotsam and jetsam coming off at the 5’ hydroxyl?” said Katherine Seley-Radtke. Among medicinal chemists, this type of protecting group is casually known as “a McGuigan protide.” Designed by medicinal chemist Chris McGuigan in the 1990s, this type of protecting group and its variations are widely used to deliver nucleotide analogs into cells.

“It is a brilliant system, because it accomplishes two things,” Seley-Radtke said. “No. 1, an issue with nucleosides is that they’re polar and their phosphates are even more polar.” Masking the highly negative phosphate groups with esters or amides reduces the molecule’s overall polarity, letting it cross the plasma membrane into cells.

Second, in order to be recognized by polymerases, the analog needs to resemble a normal nucleotide triphosphate—which means it needs to be phosphorylated.

“The first phosphorylation, either by cellular or viral kinases, is oftentimes very difficult,” Seley-Radtke said. “A lot of those kinases are very, very picky in terms of recognition.” By arriving in the cell with its first phosphate already in tow, remdesivir and related nucleotide analogs skip that rate-limiting step. After the protecting groups are cleaved, the nucleotide analog is a reasonable substrate for later nucletodie kinases.